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RWD High Activity Umbilical Cord Enzymatic Digestion Kit(Human) optimized enzymatic hydrolysis formulation for efficient and gentle digestion of human umbilical cord tissues, preserve the integrity of surface antigenic epitopes.
Cat No: DHUTE-2516
Number of reactions: 25T
Q&A:
Q1: What are the storage conditions for the tissue enzymatic digestion kit?
A1: Different kits contain different types of enzymes. Store them separately in a refrigerator at 4°C or -20°C according to the storage requirements in the instructions.
Q2: What are the precautions for the pretreatment of tissue samples?
A2: During the sampling process, carefully remove blood vessels and residual blood clots, and repeatedly rinse the tissue until it is clean.
Q3: What are the main types of target cells for extraction?
A3: Umbilical cord mesenchymal stem cells can be obtained.
Cell viability is 99.76%. MSCs (Mesenchymal Stem Cells) population is selected by surface markers CD73+, CD90+, CD45-, and CD34-.
Cells start to adhere at P0-8d. After subculture, the cell growth ability is significantly enhanced, and the cells present a uniform long spindle shape.